HPLC and UHPLC columnsEC HPLC column (analytical), NUCLEOSIL 100-5 C18, 5 µm, 250×4.6 mm


This HPLC column is packed with endcapped C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

REF 720014.46
Package unit 1 Piece(s)

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Hazardous material No
Method HPLC, analytical
Base material NUCLEOSIL – the standard silica
Phase NUCLEOSIL 100-5 C18
USP listing L1
Surface chemistry Octadecyl (C18, ODS) modification
Mode Reversed phase (RP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4.6 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) Aldehydes, Basic, neutral or acidic drugs, Derivatized amino acids, Fat-soluble vitamins, Ketones, Pesticides, Phenolic compounds, Reversed phase, RP applications HPLC
Endcapped Yes
Carbon content 15.0 %
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Pore volume 1 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 400 bar
Column volume 4.15 mL
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis


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1 kg, 5 kg, 25 kg